IPSC-derived megakaryocytes and platelets accelerate wound healing and angiogenesis.

ABSTRACT:

Background

Platelet-rich plasma (PRP), which is prepared by concentrating platelets in autologous blood, shows efficacy in chronic skin wounds via multiple growth factors. However, it exhibits heterogeneity across patients, leading to unstable therapeutic efficacy. Human induced pluripotent stem cell (iPSC)-derived megakaryocytes and platelets (iMPs) are capable of providing a stable supply, holding promise as materials for novel platelet concentrate-based therapies. In this context, we evaluated the effect of iMPs on wound healing and validated lyophilization for clinical applications.

Methods

The growth factors released by activated iMPs were measured. The effect of the administration of iMPs on human fibroblasts and human umbilical vein endothelial cells (HUVECs) was inve

SUBMITTER: Kosaka K 

PROVIDER: S-EPMC11477011 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature