Interaction of Tri-Cyclic Nucleobase Analogs with Enzymes of Purine Metabolism: Xanthine Oxidase and Purine Nucleoside Phosphorylase.

ABSTRACT: Fluorescent markers play important roles in spectroscopic and microscopic research techniques and are broadly used in basic and applied sciences. We have obtained markers with fluorescent properties, two etheno derivatives of 2-aminopurine, as follows: 1,N²-etheno-2-aminopurine (1,N²-ε2APu, I) and N²,3-etheno-2-aminopurine (N²,3-ε2APu, II). In the present paper, we investigate their interaction with two key enzymes of purine metabolism, purine nucleoside phosphorylase (PNP), and xanthine oxidase (XO), using diffraction of X-rays on protein crystals, isothermal titration calorimetry, and fluorescence spectroscopy. Crystals were obtained and structures were solved for WT PNP and D204N-PNP mutant in a complex with N²,3-ε2APu

SUBMITTER: Stachelska-Wierzchowska A 

PROVIDER: S-EPMC11477426 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature