Turn-On Fluorescence Probe for Cancer-Related γ-Glutamyltranspeptidase Detection.

ABSTRACT: The design and development of fluorescent materials for detecting cancer-related enzymes are crucial for cancer diagnosis and treatment. Herein, we present a substituted rhodamine derivative for the chromogenic and fluorogenic detection of the cancer-relevant enzyme γ-glutamyltranspeptidase (GGT). Initially, the probe is non-chromic and non-emissive due to its spirolactam form, which hinders extensive electronic delocalization over broader pathway. However, selective enzymatic cleavage of the side-coupled group triggers spirolactam ring opening, resulting in electronic flow across the rhodamine skeleton, and reduces the band gap for low-energy electronic transitions. This transformation turns the reaction mixture from colorless to intense pink, with prominent UV and fluorescence ban

SUBMITTER: Saleem M 

PROVIDER: S-EPMC11477498 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature