Dual Fractions Proteomic Analysis of Silica Nanoparticle Interactions with Protein Extracts.

ABSTRACT: Dual-fraction proteomics reveals a novel class of proteins impacted by nanoparticle exposure.

Background

Nanoparticles (NPs) interact with cellular proteomes, altering biological processes. Understanding these interactions requires comprehensive analyses beyond solely characterizing the NP corona.

Methods

We utilized a dual-fraction mass spectrometry (MS) approach to analyze both NP-bound and unbound proteins in Saccharomyces cerevisiae sp. protein extracts exposed to silica nanoparticles (SiNPs). We identified unique protein signatures for each fraction and quantified protein abundance changes using spectral counts.

Results

Strong correlations were observed between protein profiles in each fraction and non-exposed controls, while minimal correlation existed bet

SUBMITTER: Schvartz M 

PROVIDER: S-EPMC11478063 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature