Genetic Transformation of <i>Triticum dicoccum</i> and <i>Triticum aestivum</i> with Genes of Jasmonate Biosynthesis Pathway Affects Growth and Productivity Characteristics.

ABSTRACT: The transformation protocol based on the dual selection approach (fluorescent protein and herbicide resistance) has been applied here to produce transgenic plants of two cereal species, emmer wheat and bread wheat, with the goal of activating the synthesis of the stress hormone jasmonates by overexpressing ALLENE OXIDE SYNTHASE from Arabidopsis thaliana (AtAOS) and bread wheat (TaAOS) and OXOPHYTODIENOATE REDUCTASE 3 from A. thaliana (AtOPR3) under the strong constitutive promoter (ZmUbi1), either individually or both genes simultaneously. The delivery of the expression cassette encoding AOS was found to affect morphogenesis in both wheat species negatively. The effect of transgene expression on the accumulation of individual jasmonat

SUBMITTER: Miroshnichenko DN 

PROVIDER: S-EPMC11478715 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature