Seasonal environmental fluctuations alter the transcriptome dynamics of oocytes and granulosa cells in beef cows.

ABSTRACT:

Background

Examining the mechanistic cellular responses to heat stress could aid in addressing the increasing prevalence of decreased fertility due to elevated ambient temperatures. Here, we aimed to study the differential responses of oocytes and granulosa cells to thermal fluctuations due to seasonal differences. Dry beef cows (n = 10) were housed together, synchronized and subjected to a stimulation protocol to induce follicular growth before ovum pick-up (OPU). Two OPU's were conducted (summer and winter) to collect cumulus-oocyte-complexes (COCs) and granulosa cells. In addition, rectal temperatures and circulating blood samples were collected during OPU. Oocytes were separated from the adherent cumulus cells, and granulosa cells were isolated from the collected OPU fluid. RNA

SUBMITTER: Joyce K 

PROVIDER: S-EPMC11479552 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature