M protein ectodomain-specific immunity restrains SARS-CoV-2 variants replication.

ABSTRACT:

Introduction

The frequent occurrence of mutations in the SARS-CoV-2 Spike (S) protein, with up to dozens of mutations, poses a severe threat to the current efficacy of authorized COVID-19 vaccines. Membrane (M) protein, which is the most abundant viral structural protein, exhibits a high level of amino acid sequence conservation. M protein ectodomain could be recognized by specific antibodies; however, the extent to which it is immunogenic and provides protection remains unclear.

Methods

We designed and synthesized multiple peptides derived from coronavirus M protein ectodomains, and determined the secondary structure of specific peptides using circular dichroism (CD) spectroscopy. Enzyme-linked immunosorbent assay (ELISA) was utilized to detect IgG responses against the syn

SUBMITTER: Tang Y 

PROVIDER: S-EPMC11480003 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature