Comparative analysis of porcine-uterine decellularization for bioactive-molecule preservation and DNA removal.

ABSTRACT:

Introduction

Decellularized uterine extracellular matrix has emerged as a pivotal focus in the realm of biomaterials, offering a promising source in uterine tissue regeneration, research on disease diagnosis and treatments, and ultimately uterine transplantation. In this study, we examined various protocols for decellularizing porcine uterine tissues, aimed to unravel the intricate dynamics of DNA removal, bioactive molecules preservation, and microstructural alterations.

Methods

Porcine uterine tissues were treated with 6 different, yet rigorously selected and designed, protocols with sodium dodecyl sulfate (SDS), Triton^® X-100, peracetic acid + ethanol, and DNase I. After decellularization, we examined DNA quantification, histological staining (H&E and DAPI), gl

SUBMITTER: Fazel Anvari Yazdi A 

PROVIDER: S-EPMC11480021 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature