3D-Aligner: advanced computational tool for correcting image distortion in expansion microscopy.

ABSTRACT: Expansion Microscopy (ExM) is an innovative and cost-effective super-resolution microscopy technique that has become popular in cell biology research. It achieves super-resolution by physically expanding specimens. Since its introduction, ExM has undergone continuous methodological developments to enhance its resolution and labeling capabilities. However, ExM imaging often encounters sample drift during image acquisition due to the physical movement of the expanded hydrogel, posing a significant challenge for accurate image reconstruction. Despite many proposed experimental solutions to mitigate sample drift, a universal solution has yet to be established. In response to this challenge, we developed 3D-Aligner, an advanced and user-friendly image analysis tool designed to computationally c

SUBMITTER: Loi J 

PROVIDER: S-EPMC11480096 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature