High sensitivity cameras can lower spatial resolution in high-resolution optical microscopy.

ABSTRACT: High-resolution optical fluorescence microscopies and, in particular, super-resolution fluorescence microscopy, are rapidly adopting highly sensitive cameras as their preferred photodetectors. Camera-based parallel detection facilitates high-speed live cell imaging with the highest spatial resolution. Here, we show that the drive to use ever more sensitive, photon-counting image sensors in cameras can, however, have detrimental effects on the spatial resolution of the resulting images. This is particularly noticeable in applications that demand a high space-bandwidth product, where the image magnification is close to the Nyquist sampling limit of the sensor. Most scientists will often select image sensors based on parameters such as pixel size, quantum efficiency, signal-to-noise performan

SUBMITTER: Ortkrass H 

PROVIDER: S-EPMC11480200 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature