Precision comparison of intensity ratios and area ratios in spectral analysis.

ABSTRACT: The long-debated question in analytical chemistry of which of the area ratio or the intensity ratio is the more precise has yielded no definitive analytical conclusion. To address this issue theoretically, we derived analytical solutions for the lower limits of estimation precision for spectral parameters, including the intensity ratio and area ratio, based on the Cramér-Rao lower bound (CRLB) framework for a Gaussian spectrum. The precisions of spectral parameter estimations from the analytical solutions were consistent with results obtained from Monte Carlo simulations. Our theoretical and simulation results revealed that the precision of estimating the area ratio surpassed that of the intensity ratio by a factor of 2 . Additionally, our experimental results aligned well with both theore

SUBMITTER: Hagiwara Y 

PROVIDER: S-EPMC11480322 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature