Identification of circRNA CDR1as/miR-214-3p regulatory axis in Legg-Calve-Perthes disease.

ABSTRACT:

Background

Legg-Calvé-Perthes disease (LCPD) commonly occurs among adolescents, threatening their health. However, the potential mechanism underlying LCPD remains unclear. miR-214-3p is shown as a critical role in LCPD development with unspecified upstream regulators.

Methods

Levels of miR-214-3p and circCDR1as in healthy controls and LCPD patients were determined by qRT-PCR. The role of circCDR1as/miR-214-3p axis in LCPD was determined by testing the cell viability and apoptosis in TC28 cells and primary chondrocytes. Regulation between circCDR1as and miR-214-3p was examined by RIP and ChIP assays. The inflammatory response and angiogenesis were evaluated by M2 macrophage polarization and HUVECs tumor formation.

Results

circCDR1as was overexpressed in LCPD patients w

SUBMITTER: Lan X 

PROVIDER: S-EPMC11481470 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature