The role of DNA methylation in chondrogenesis of human iPSCs as a stable marker of cartilage quality.

ABSTRACT:

Background

Lack of insight into factors that determine purity and quality of human iPSC (hiPSC)-derived neo-cartilage precludes applications of this powerful technology toward regenerative solutions in the clinical setting. Here, we set out to generate methylome-wide landscapes of hiPSC-derived neo-cartilages from different tissues-of-origin and integrated transcriptome-wide data to identify dissimilarities in set points of methylation with associated transcription and the respective pathways in which these genes act.

Methods

We applied in vitro chondrogenesis using hiPSCs generated from two different tissue sources: skin fibroblasts and articular cartilage. Upon differentiation toward chondrocytes, these are referred to as hFiCs and hCiC, respectively. Genome-wide DNA methy

SUBMITTER: Hajmousa G 

PROVIDER: S-EPMC11481477 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature