Rubella virus assembly requirements and evolutionary relationships with novel rubiviruses.

ABSTRACT: Rubella virus (RuV) is an enveloped virus that usually causes mild disease in children, but can produce miscarriage or severe congenital birth defects. While in nature RuV only infects humans, the discovery of the related Ruhugu (RuhV) and Rustrela (RusV) viruses highlights the spillover potential of mammalian rubiviruses to humans. RuV buds into the Golgi, but its assembly and exit are not well understood. We identified a potential late domain motif ²⁷⁸PPAY²⁸¹ at the C-terminus of the RuV E2 envelope protein. Such late domain motifs can promote virus budding by recruiting the cellular ESCRT machinery. An E2 Y281A mutation reduced infectious virus production by >3 logs and inhibited virus particle production. However, RuV was insensitive to inhibition by dominant-nega

SUBMITTER: Das PK 

PROVIDER: S-EPMC11481484 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature