Performance of MALDI-TOF MS, real-time PCR, antigen detection, and automated biochemical testing for the identification of <i>Burkholderia pseudomallei</i>.

ABSTRACT: Burkholderia pseudomallei is the causative agent of melioidosis, a disease highly endemic to Southeast Asia and northern Australia, though the area of endemicity is expanding. Cases may occur in returning travelers or, rarely, from imported contaminated products. Identification of B. pseudomallei is challenging for laboratories that do not see this organism frequently, and misidentifications by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) and automated biochemical testing have been reported. The in vitro diagnostic database for use with the Vitek MS has recently been updated to include B. pseudomallei and we aimed to validate the performance for identification in comparison to automated biochemical testing with the

SUBMITTER: Campbell S 

PROVIDER: S-EPMC11481520 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature