FabR, a regulator of membrane lipid homeostasis, is involved in <i>Klebsiella pneumoniae</i> biofilm robustness.

ABSTRACT: Biofilm is a dynamic structure from which individual bacteria and micro-aggregates are released to subsequently colonize new niches by either detachment or dispersal. Screening of a transposon mutant library identified genes associated with the alteration of Klebsiella pneumoniae biofilm including fabR, which encodes a transcriptional regulator involved in membrane lipid homeostasis. An isogenic ∆fabR mutant formed more biofilm than the wild-type (WT) strain and its trans-complemented strain. The thick and round aggregates observed with ∆fabR were resistant to extensive washes, unlike those of the WT strain. Confocal microscopy and BioFlux microfluidic observations showed that fabR deletion was associated with biofilm robustness and impaired erosion over

SUBMITTER: Drame I 

PROVIDER: S-EPMC11481535 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature