<i>Anaplasma phagocytophilum</i> invasin AipA interacts with CD13 to elicit Src kinase signaling that promotes infection.

ABSTRACT: Host-microbe interactions that facilitate entry into mammalian cells are essential for obligate intracellular bacterial survival and pathogenesis. Anaplasma phagocytophilum is an obligate intracellular bacterium that invades neutrophils to cause granulocytic anaplasmosis. The invasin-receptor pairs and signaling events that induce Anaplasma uptake are inadequately defined. A. phagocytophilum invasion protein A orchestrates entry via residues 9-21 (AipA_9-21) engaging an unknown receptor. Yeast two-hybrid screening suggested that AipA binds within C-terminal amino acids 851-967 of CD13 (aminopeptidase N), a multifunctional protein that, when crosslinked, initiates Src kinase and Syk signaling that culminates in endocytosis. Co-immunoprecipitation validated the

SUBMITTER: Lind MCH 

PROVIDER: S-EPMC11481542 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature