Cell shape and division septa positioning in filamentous <i>Streptomyces</i> require a functional cell wall glycopolymer ligase CglA.

ABSTRACT: The cell wall of monoderm bacteria consists of peptidoglycan and glycopolymers in roughly equal proportions and is crucial for cellular integrity, cell shape, and bacterial vitality. Despite the immense value of Streptomyces in biotechnology and medicine as antibiotic producers, we know very little about their cell wall biogenesis, composition, and functions. Here, we have identified the LCP-LytR_C domain protein CglA (Vnz_13690) as a key glycopolymer ligase, which specifically localizes in zones of cell wall biosynthesis in S. venezuelae. Reduced amount of glycopolymers in the cglA mutant results in enlarged vegetative hyphae and failures in FtsZ-rings formation and positioning. Consequently, division septa are misplaced leading to the formation of aberrant cel

SUBMITTER: Bhowmick S 

PROVIDER: S-EPMC11481543 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature