Structural analysis of S-ring composed of FliFG fusion proteins in marine Vibrio polar flagellar motor.

ABSTRACT: The marine bacterium Vibrio alginolyticus possesses a polar flagellum driven by a sodium ion flow. The main components of the flagellar motor are the stator and rotor. The C-ring and MS-ring, which are composed of FliG and FliF, respectively, are parts of the rotor. Here, we purified an MS-ring composed of FliF-FliG fusion proteins and solved the near-atomic resolution structure of the S-ring-the upper part of the MS-ring-using cryo-electron microscopy. This is the first report of an S-ring structure from Vibrio, whereas, previously, only those from Salmonella have been reported. The Vibrio S-ring structure reveals novel features compared with that of Salmonella, such as tilt angle differences of the RBM3 domain and the β-collar region, which contribute t

SUBMITTER: Takekawa N 

PROVIDER: S-EPMC11481574 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature