Exogenous hydrogen sulfide enhances myogenic differentiation of C2C12 myoblasts under high palmitate stress.

ABSTRACT: Skeletal muscle atrophy was one of main complications of type 2 diabetes mellitus. Hydrogen sulfide (H₂S) is involved in various physiological functions, such as anti-hypertension and anti-oxidant. Skeletal muscle atrophy caused by type 2 diabetes could lead to the regeneration of muscle fibers. Wnt signaling pathway plays a crucial important role in this process. H₂S maybe regulate the Wnt signaling pathway to alleviate skeletal muscle atrophy, however, this role has not been clarified. The aim of this study is to investigate the potential regulatory role of H₂S in the Wnt signaling pathway. C2C12 myoblasts treated with 500 μmol palmitate as an in vitro model. Western blot was used to detect the levels of CSE, PKM1, β-catenin, MuRF1, MYOG, MYF6 and MYOD1.

SUBMITTER: Lu F 

PROVIDER: S-EPMC11481675 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature