The RhoGEF protein Plekhg5 self-associates via its PH domain to regulate apical cell constriction.

ABSTRACT: RhoGEFs are critical activators of Rho family small GTPases and regulate diverse biological processes, such as cell division and tissue morphogenesis. We reported previously that the RhoGEF gene plekhg5 controls apical constriction of bottle cells at the blastopore lip during Xenopus gastrulation, but the detailed mechanism of plekhg5 action is not understood in depth. In this study, we show that localization of Plekhg5 in the apical cortex depends on its N-terminal sequences and intact guanine nucleotide exchange activity, whereas the C-terminal sequences prevent ectopic localization of the protein to the basolateral compartment. We also reveal that Plekhg5 self-associates via its PH domain, and this interaction leads to functional rescue of two mutants that lack the

SUBMITTER: Popov IK 

PROVIDER: S-EPMC11481697 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature