Microcavity-assisted cloning (MAC) of hard-to-clone HepG2 cell lines: cloning made easy.

ABSTRACT: Cloning is a key molecular biology procedure for obtaining a genetically homogenous population of organisms or cell lines. It requires the expansion of new cell populations starting from single genetically modified cells. Despite the technical progress, cloning of many cell lines remains difficult. Cloning often fails either due to the strenuous conditions associated with manipulating cells or because many cells don't tolerate a single-cell state. Here we describe a new cloning method utilizing low adhesion microcavity plates. This new technique, named microcavity-assisted cloning (MAC) was developed to clone difficult-to-clone HepG2 cells. The clones were produced following CRISPR/Cas9 knockout of the GSTA1 gene by a random distribution of 200, 400, and 800 cells into 550 microcavities of

SUBMITTER: Mlakar V 

PROVIDER: S-EPMC11481743 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature