A growth-coupling strategy for improving the stability of terpenoid bioproduction in Escherichia coli.

ABSTRACT:

Background

Achieving cost-competitiveness remains challenging for industrial biomanufacturing. With whole-cell biocatalysis, inefficiency presents when individual cells vary in their production levels. The problem exacerbates when the basis for such production heterogeneity is heritable. Here, evolution selects for the low- and non-producers, as they have lowered/abolished the cost of bioproduction to fitness. With the scale of population expansion required for industrial bioproduction, the asymmetrical enrichment can be severe enough to compromise the performance, and hence commercial viability of the bioprocess. Clearly, addressing production heterogeneity is crucial, especially in improving the stability of bioproduction across the cell generations. In this respect, we designed

SUBMITTER: Tan JC 

PROVIDER: S-EPMC11481808 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature