Visualization of Rostral Migratory Stream in the Developing Rat Brain by In Vivo Electroporation.

ABSTRACT: Interneurons in the olfactory bulb (OB) are generated from neuronal precursor cells migrating from anterior subventricular zone (SVZa) not only in the developing embryo but also throughout the postnatal life of mammals. In the present study, we established an in vivo electroporation assay to label SVZa cells of rat both at embryonic and postnatal ages, and traced SVZa progenitors and followed their migration pathway and differentiation. We found that labeled cells displayed high motility. Interestingly, the postnatal cells migrated faster than the embryonic cells after applying this assay at different ages of brain development. Furthermore, based on brain slice culture and time-lapse imaging, we analyzed the detail migratory properties of these labeled precursor neurons. Finally, tissue tr

SUBMITTER: Xie YW 

PROVIDER: S-EPMC11481847 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature