Directly monitoring the dynamic in vivo metabolisms of hyperpolarized ¹³C-oligopeptides.

ABSTRACT: Peptides play essential roles in biological phenomena, and, thus, there is a growing interest in detecting in vivo dynamics of peptide metabolisms. Dissolution-dynamic nuclear polarization (d-DNP) is a state-of-the-art technology that can markedly enhance the sensitivity of nuclear magnetic resonance (NMR), providing metabolic and physiological information in vivo. However, the hyperpolarized state exponentially decays back to the thermal equilibrium, depending on the spin-lattice relaxation time (T₁). Because of the limitation in T₁, peptide-based DNP NMR molecular probes applicable in vivo have been limited to amino acids or dipeptides. Here, we report the direct detection of in vivo metabolic conversions of hyperpolarized ¹³C-oligopeptides.

SUBMITTER: Kondo Y 

PROVIDER: S-EPMC11482307 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature