Tn4661-mediated transfer of bla _CTX-M-15 from Klebsiella michiganensis to an outbreak clone of Pseudomonas aeruginosa.

ABSTRACT: Carriage of CTX-M-type extended-spectrum β-lactamase (ESBL) is rare in Pseudomonas aeruginosa. During routine surveillance of an endemic ST-621 P. aeruginosa at a large hospital, isolate MRSN 100690 carrying bla _CTX-M-15 was cultured from a patient (P2). This was the first detection of this ESBL in the endemic ST-621 lineage. All 1 488 bacterial isolates collected from the same facility in the 12 months prior to the incidence of 100 690 were screened for the presence of bla _CTX-M-15. A set of 183 isolates was identified, in which corresponding patient metadata was evaluated for spatiotemporal overlaps with P2. The resulting three isolates, along with 100 690, were long-read sequenced using the Oxford Nanopore MinION platform to determine a

SUBMITTER: Bartlett KV 

PROVIDER: S-EPMC11482538 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature