MiR-27a inhibits the growth and metastasis of multiple myeloma through regulating Th17/Treg balance.

ABSTRACT:

Background

The imbalance between T helper 17 (Th17) and T regulatory (Treg) cells plays a key role in the progression of multiple myeloma (MM).

Methods

The gene expression profiles of MM were acquired and examined from the Gene Expression Omnibus (GEO) database (GSE72213). Our research involved experimental investigations conducted using the MOPC-MM mouse model. Dysregulation of Treg and Th17 cells was evaluated through flow cytometry, while the levels of inflammatory factors were measured using the enzyme-linked immunosorbent assay. Cell proliferation was gauged using the Cell Counting Kit-8 assay, and cell apoptosis was quantified via flow cytometry. Cell metastasis capabilities were determined by conducting transwell assays. To confirm the relationship between miR-27a and

SUBMITTER: Lu W 

PROVIDER: S-EPMC11482689 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature