Dynamic barriers modulate cohesin positioning and genome folding at fixed occupancy.

ABSTRACT: In mammalian interphase cells, genomes are folded by cohesin loop extrusion limited by directional CTCF barriers. This interplay leads to the enrichment of cohesin at barriers, isolation between neighboring topologically associating domains, and elevated contact frequency between convergent CTCF barriers across the genome. However, recent in vivo measurements present a puzzle: reported residence times for CTCF on chromatin are in the range of a few minutes, while lifetimes for cohesin are much longer. Can the observed features of genome folding result from the action of relatively transient barriers? To address this question, we developed a dynamic barrier model, where CTCF sites switch between bound and unbound states with rates that can be directly compared with biophysical measur

SUBMITTER: Rahmaninejad H 

PROVIDER: S-EPMC11482749 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature