Application of a new highly multiplexed amplicon sequencing tool to evaluate <i>Plasmodium falciparum</i> antimalarial resistance and relatedness in individual and pooled samples from Dschang, Cameroon.

ABSTRACT:

Background

Resistance to antimalarial drugs remains a major obstacle to malaria elimination. Multiplexed, targeted amplicon sequencing is being adopted for surveilling resistance and dissecting the genetics of complex malaria infections. Moreover, genotyping of parasites and detection of molecular markers drug resistance in resource-limited regions requires open-source protocols for processing samples, using accessible reagents, and rapid methods for processing numerous samples including pooled sequencing.

Methods

P lasmodium f alciparum Streamlined Multiplex Antimalarial Resistance and Relatedness Testing (Pf-SMARRT) is a PCR-based amplicon panel consisting of 15 amplicons targeting antimalarial resistance mutations and 9 amplicons targeti

SUBMITTER: Sadler JM 

PROVIDER: S-EPMC11482863 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature