Microscopic Phage Adsorption Assay: High-throughput quantification of virus particle attachment to host bacterial cells.

ABSTRACT: Phages, viruses of bacteria, play a pivotal role in Earth's biosphere and hold great promise as therapeutic and diagnostic tools in combating infectious diseases. Attachment of phages to bacterial cells is a crucial initial step of the interaction. The classic assay to quantify the dynamics of phage attachment involves co-culturing and enumeration of bacteria and phages, which is laborious, lengthy, hence low-throughput, and only provides ensemble estimates of model-based adsorption rate constants. Here, we utilized fluorescence microscopy and particle tracking to obtain trajectories of individual virus particles interacting with cells. The trajectory durations quantified the heterogeneity in dwell time, the time that each phage spends interacting with a bacterium. The average dwell time s

SUBMITTER: Antani JD 

PROVIDER: S-EPMC11482966 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature