Negative regulation of APC/C activation by MAPK-mediated attenuation of Cdc20^Slp1 under stress.

ABSTRACT: Mitotic anaphase onset is a key cellular process tightly regulated by multiple kinases. The involvement of mitogen-activated protein kinases (MAPKs) in this process has been established in Xenopus egg extracts. However, the detailed regulatory cascade remains elusive, and it is also unknown whether the MAPK-dependent mitotic regulation is evolutionarily conserved in the single-cell eukaryotic organisms such as fission yeast (Schizosaccharomyces pombe). Here, we show that two MAPKs in S. pombe indeed act in concert to restrain anaphase-promoting complex/cyclosome (APC/C) activity upon activation of the spindle assembly checkpoint (SAC). One MAPK, Pmk1, binds to and phosphorylates Slp1^Cdc20, the co-activator of APC/C. Phosphorylation of Slp1^Cdc20 by

SUBMITTER: Sun L 

PROVIDER: S-EPMC11483130 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature