Biosynthesis of Halogenated Tryptophans for Protein Engineering Using Genetic Code Expansion.

ABSTRACT: Genetic Code Expansion technology offers significant potential in incorporating noncanonical amino acids into proteins at precise locations, allowing for the modulation of protein structures and functions. However, this technology is often limited by the need for costly and challenging-to-synthesize external noncanonical amino acid sources. In this study, we address this limitation by developing autonomous cells capable of biosynthesizing halogenated tryptophan derivatives and introducing them into proteins using Genetic Code Expansion technology. By utilizing inexpensive halide salts and different halogenases, we successfully achieve the selective biosynthesis of 6-chloro-tryptophan, 7-chloro-tryptophan, 6-bromo-tryptophan, and 7-bromo-tryptophan. These derivatives are introduced at speci

SUBMITTER: Guo Y 

PROVIDER: S-EPMC11483216 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature