Visual and fluorescent detection of Moraxella catarrhalis by multiple cross displacement amplification-based assay.

ABSTRACT: Moraxella catarrhalis (M. catarrhalis), once considered as a harmless commensal bacterium, is now recognized as a significant pathogen associated with human mucosal infection. Hence, development of rapid, accurate, and user-friendly detection method is crucial for effective control and management of M. catarrhalis infections. Here, we introduce the M. catarrhalis MCDA-FRT assay, a novel diagnostic approach based on multiple cross displacement amplification (MCDA) for the visual and fluorescent (FRT) detection of M. catarrhalis. This innovative method amplifies the target sequence through MCDA reaction and provides results through the visual observation of a fluorescent signal when exposed to blue light. The generation of the fluorescent signal is facilita

SUBMITTER: Huang X 

PROVIDER: S-EPMC11483306 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature