Isolation of CD63-positive epididymosomes from human semen and its application in improving sperm function.

ABSTRACT: Extracellular vesicles (EVs) are highly heterogeneous, and different EV subpopulations from various origins mediate different biological effects. The separation of different subpopulations of EVs from mixtures is critical but challenging. Epididymosomes are secreted by the epididymal epithelium and play a key role in sperm maturation and function. However, limited access to human epididymal tissue and epididymal fluid has hampered further study of epididymosomes and their potential clinical applications. Here, we established a novel strategy based on flow cytometry sorting to isolate human CD63-positive epididymosomes from ejaculate. We identified CD52, a membrane-located protein expressed exclusively in the epididymis, as the sorting marker for human epididymosomes. Then, CD63-positive ep

SUBMITTER: Luo J 

PROVIDER: S-EPMC11483612 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature