High-Speed Imaging of Giant Unilamellar Vesicle Formation in cDICE.

ABSTRACT: Giant unilamellar vesicles (GUVs) are widely used as in vitro model membranes in biophysics and as cell-sized containers in synthetic biology. Despite their ubiquitous use, there is no one-size-fits-all method for their production. Numerous methods have been developed to meet the demanding requirements of reproducibility, reliability, and high yield while simultaneously achieving robust encapsulation. Emulsion-based methods are often praised for their apparent simplicity and good yields; hence, methods like continuous droplet interface crossing encapsulation (cDICE), which make use of this principle, have gained popularity. However, the underlying physical principles governing the formation of GUVs in cDICE and related methods remain poorly understood. To this end, we have developed a high

SUBMITTER: Van de Cauter L 

PROVIDER: S-EPMC11483911 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature