Uncovering the fragmentation and separation characteristics of sophorolipid biosurfactants with LC-MS-ESI.

ABSTRACT: The application of liquid chromatography and mass spectrometry (MS) is a challenging area of research for structural identification of sophorolipids, owing to the large number of possible variations in structure and limited knowledge on the separation and fragmentation characteristics of the variants. The aims of this work was to provide a comprehensive analysis of the expected characteristics and fragmentation patterns of a wide range of sophorolipid biosurfactant congeners, providing a methodology and process alongside freely available data to inform and enable future research of commercial or novel sophorolipids. Samples of acidic and lactonic sophorolipid standards were tested using reverse-phase ultra-high performance liquid chromatography and identified using electrospray ionization

SUBMITTER: Ingham B 

PROVIDER: S-EPMC11484030 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature