Mechanism of ginsenoside Rb₃ against OGD/R damage based on metabonomic and PCR array analyses.

ABSTRACT: In order to study the mechanisms of ginsenoside Rb₃ (G-Rb₃) against oxygen-glucose deprivation/reoxygenation (OGD/R) injury in HT22 cells based on metabolomics and PCR array, HT22 cells were randomly divided into control group, model group, G-Rb₃ high-dose group (10 µmol/l) and G-Rb₃ low-dose group (5 µmol/l). Except for the control group, which was left untreated, the remaining groups were incubated with 10 mmol/l Na₂S₂O₄ in sugar-free DMEM medium for 2 h and then replaced with serum-free high-sugar DMEM medium for 2 h in order to replicate in vitro OGD/R model. Trypan blue staining was used to detect the cell viability; flow cytometry was used to detect apoptosis; western blotting was used to detect the p

SUBMITTER: Li F 

PROVIDER: S-EPMC11484189 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature