Effects of inorganic phosphate on stem cells isolated from human exfoliated deciduous teeth.

ABSTRACT: Calcium phosphate-based materials (CaP) are introduced as potential dental pulp capping materials for deciduous teeth. The present study investigated the influence of inorganic phosphate (P_i) on regulating stem cells isolated from human exfoliated deciduous teeth (SHED). SHEDs were treated with P_i. Cell cycle progression and apoptosis were examined using flow cytometry analysis. Osteo/odontogenic and adipogenic differentiation were analyzed using alizarin red S and oil red O staining, respectively. The mRNA expression profile was investigated using a high-throughput RNA sequencing technique. P_i increased the late apoptotic cell population while cell cycle progression was not altered. P_i upregulated osteo/odontoblastic gene expression and enhanced

SUBMITTER: Suwittayarak R 

PROVIDER: S-EPMC11484878 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature