DoE-assisted HPLC method development and validation of enzalutamide and repaglinide in rat plasma.

ABSTRACT: Aim: A simple and rapid HPLC technique was developed and validated to simultaneously estimate enzalutamide (ENZ) and repaglinide (REP) in rat plasma.Methods: In silico predictions using DDinter and DDI-Pred indicated possible drug-drug interactions between ENZ and REP. A central composite design was used to identify factors influencing the separation of the drugs. Interactions between chromatographic parameters were studied through 51 experiments, followed by illustration with three-dimensional response surface plots. The four factors optimized for the separation of the two drugs are column temperature (A), % organic strength (B), pH (C) and column type (D).Results: Plate count(R1), tailing factor (R2) and resolution (R3) responses in the experimental design wer

SUBMITTER: Reddy GN 

PROVIDER: S-EPMC11486095 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature