Context effects on repair of 5'-overhang DNA double-strand breaks induced by Cas12a in Arabidopsis.

ABSTRACT: Sequence-specific endonucleases have been key to the study of the mechanisms and control of DNA double-strand break (DSB) repair and recombination, and the availability of CRISPR-Cas nucleases over the last decade has driven rapid progress in the understanding and application of targeted recombination in many organisms, including plants. We present here an analysis of recombination at targeted chromosomal 5' overhang DSB generated by the FnCas12a endonuclease in the plant, Arabidopsis thaliana. The much-studied Cas9 nuclease cleaves DNA to generate blunt-ended DSBs, but relatively less is known about the repair of other types of breaks, such as those with 5'-overhanging ends. Sequencing the repaired breaks clearly shows that the majority of repaired DSB carry small deletions and are

SUBMITTER: Lageix S 

PROVIDER: S-EPMC11486519 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature