A modular DNA origami nanocompartment for engineering a cell-free, protein unfolding and degradation pathway.

ABSTRACT: Within the cell, chemical reactions are often confined and organized through a modular architecture. This facilitates the targeted localization of molecular species and their efficient translocation to subsequent sites. Here we present a cell-free nanoscale model that exploits compartmentalization strategies to carry out regulated protein unfolding and degradation. Our synthetic model comprises two connected DNA origami nanocompartments (each measuring 25 nm × 41 nm × 53 nm): one containing the protein unfolding machine, p97, and the other housing the protease chymotrypsin. We achieve the unidirectional immobilization of p97 within the first compartment, establishing a gateway mechanism that controls substrate recruitment, translocation and processing within the second compartment. Our dat

SUBMITTER: Huang J 

PROVIDER: S-EPMC11486656 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature