Development of a loop-mediated isothermal amplification detection assay for <i>Dictyocaulus viviparus</i> (Bloch, 1782) lungworm: DviLAMP.

ABSTRACT: The bovine lungworm, Dictyocaulus viviparus (Bloch, 1782), is highly pathogenic and disease outbreaks can be difficult to predict and manage. Rapid and accurate diagnosis is vital, but without a sensitive diagnostic test this remains challenging in clinical practice. High performance molecular detection tools are therefore required to improve the diagnosis of this parasite and promote the implementation of strategic control measures. Loop-mediated isothermal amplification (LAMP), a rapid DNA assay, offers potential for field-based detection. Here we report a novel LAMP assay (DviLAMP), that was designed to target the D. viviparus internal transcribed spacer 2 (ITS2) ribosomal DNA region. Firstly, genomic DNA was extracted from a single D. viviparus L₁ larva

SUBMITTER: Nak-On S 

PROVIDER: S-EPMC11486680 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature