Identification of Rv1133c (MetE) as a marker of <i>Mycobacterium tuberculosis</i> replication and as a highly immunogenic antigen with potential immunodiagnostic power.

ABSTRACT: The immunization of mice with the sterile culture medium supernatants of Mycobacterium tuberculosis (Mtb) H37Rv permitted the production of several monoclonal antibodies (mAbs) specific for secreted and/or released antigens. Two mAbs bound and immunoprecipitated an 80-kDa protein that was identified by mass spectrometry as Rv1133c, the methionine synthase MetE. The protein MetE is ubiquitous among prokaryota and shows a significant sequence homology in many bacteria. We produced both the full-length recombinant MetE and its N-terminal fragment, whose sequence is more conserved among mycobacteria, to select mAbs recognizing an Mtb-specific region of MetE. Finally, we produced and selected eight mAbs that specifically detect the MetE protein in the supernatant and cell lysate of Mtb a

SUBMITTER: Iacobino A 

PROVIDER: S-EPMC11486704 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature