Deer antler stem cells immortalization by modulation of hTERT and the small extracellular vesicles characters.

ABSTRACT:

Background

Deer antler stem cells (AnSCs) exhibit properties of both embryonic and mesenchymal stem cells, with superior self-renewal and proliferation, which drive rapid antler growth and regeneration. AnSCs and their derived small extracellular vesicles (sEVs) hold promising potential for applications in regeneration medicine. Due to the restricted proliferative capacity inherent in primary cells, the production capacity of AnSCs and their sEVs are limited. Human telomerase reverse transcriptase (hTERT) is the most important telomerase subunit, hTERT gene insertion has been successfully employed in generating immortalized cell lines.

Results

In this study, we successfully established immortalized AnSCs by transducing the hTERT gene using lentivirus. Compared to primary AnS

SUBMITTER: Chen Z 

PROVIDER: S-EPMC11486761 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature