Clinical Severity of Enteric Viruses Detected Using a Quantitative Molecular Assay Compared With Conventional Assays in the Global Enteric Multicenter Study.

ABSTRACT:

Background

Quantitative molecular assays are increasingly used for detection of enteric viruses.

Methods

We compared the clinical severity using the modified Vesikari score (mVS) of enteric viruses detected by conventional assays (enzyme immunoassays [EIAs] for rotavirus and adenovirus 40/41 and conventional polymerase chain reaction for astrovirus, sapovirus, and norovirus) and a quantitative molecular assay (TaqMan Array Card [TAC]) among children aged 0-59 months in the Global Enteric Multicenter Study. For rotavirus and adenovirus 40/41, we compared severity between EIA-positive and TAC-positive cases assigned etiologies using different cycle threshold (Ct) cutoffs.

Results

Using conventional assays, the median mVS (interquartile range) was 10 (8-11) for rotavirus

SUBMITTER: Cates J 

PROVIDER: S-EPMC11486833 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature