Agrobacterium virulence factors induce the expression of host DNA repair-related genes without promoting major genomic damage.

ABSTRACT: This study aimed to investigate whether the plant DNA damage levels and DNA damage response (DDR) are regulated during Agrobacterium infection and potentially manipulated by Agrobacterium to facilitate T-DNA integration. We investigated the plant genomic response to Agrobacterium infection by measuring gamma H2AX levels, which reflect the levels of double-strand DNA breaks (DSBs), and by characterizing transcription of three major DNA repair marker genes NAC82, KU70, and AGO2. These experiments revealed that, globally, Agrobacterium infection did not result in a major increase in DSB content in the host genome. The transcription of the DNA damage repair genes, on the other hand, was elevated upon the wild-type Agrobacterium infection. This transcriptional outcome was largely negated by a m

SUBMITTER: Lacroix B 

PROVIDER: S-EPMC11487168 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature