Differentiation of oligosaccharide isomers by direct infusion multidimensional mass spectrometry.

ABSTRACT: Oligosaccharides demonstrate many bioactivities with applications in the pharmaceutical, cosmetic, and food industries. They also serve as biomarkers for various diseases including cancer and glycogen storage disorders. These make the structural characterization of oligosaccharides very important. Unfortunately, the structural diversity found in saccharides make their characterization challenging, necessitating the development of sophisticated instrumentation to enable isomer differentiation. Herein, we report the ability of halide (Cl^- and Br^-) adducts to enable direct differentiation of oligosaccharide isomers using conventional collision-induced dissociation (CID) tandem MS (MS/MS). The halide adducts were generated by direct infusion nano-electrospray ionization (

SUBMITTER: Amoah E 

PROVIDER: S-EPMC11487224 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature