Monitoring the Dilution of Buffer Solutions with Different pH Values above and below Physiological pH in Very Small Volumes.

ABSTRACT: The accurate determination of the post-dilution concentration of biological buffers is essential for retaining the necessary properties and effectiveness of the buffer to maintain stable cellular environments and optimal conditions for biochemical reactions. In this work, we introduce a silicon-based impedance chip, which offers a rapid and reagent-free approach for monitoring the buffer concentrations after dilution with deionized (DI) water. The impedance of the impedance chip is measured, and the impedance data are modeled using a multiparameter equivalent circuit model. We investigated six aqueous biological buffers with pH values above and below the physiological pH for most tissues (pH ~ 7.2-7.4) following dilution with DI water by factors of 2.0, 10.0, 20.0, 100.0, and 200.0. The im

SUBMITTER: Bhat VJ 

PROVIDER: S-EPMC11487416 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature