Increasing Vitamin K₂ Synthesis in Bacillus subtilis by Controlling the Expression of MenD and Stabilizing MenA.

ABSTRACT: As an indispensable member of the family of lipid vitamins, vitamin K₂ (MK-7) plays an important role in blood coagulation, cardiovascular health, and kidney health. Microbial fermentation is favored due to its high utilization rate of raw materials, simple operation, and moderate conditions. However, the biosynthesis pathway of vitamin K₂ in microorganisms is highly complex, which hinders its industrial production in microbial cell factories. One of the major challenges is the stable expression and deregulation of key enzymes in the vitamin K₂ biosynthesis pathway, which remains unclear and has undergone little investigation. In this study, 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase (MenD) and 1,4-dihydroxy-2-naphthoate pol

SUBMITTER: Huang W 

PROVIDER: S-EPMC11487629 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature