A Transfer Hydrogenation Approach to Activity-Based Sensing of Formate in Living Cells.

ABSTRACT: Formate is a major reactive carbon species in one-carbon metabolism, where it serves as an endogenous precursor for amino acid and nucleic acid biosynthesis and a cellular source of NAD(P)H. On the other hand, aberrant elevations in cellular formate are connected to progression of serious diseases, including cancer and Alzheimer's disease. Traditional methods for formate detection in biological environments often rely on sample destruction or extensive processing, resulting in a loss of spatiotemporal information. To help address these limitations, here we present the design, synthesis, and biological evaluation of a first-generation activity-based sensing system for live-cell formate imaging that relies on iridium-mediated transfer hydrogenation chemistry. Formate facilitates an aldehyde-

SUBMITTER: Crossley SWM 

PROVIDER: S-EPMC11487638 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature